Hisashi MATSUDA*, Hiroki HATA, Kaoru SUGAWARA, Fengming XU, Souichi NAKASHIMA, Masayuki YOSHIKAWA, and Seikou NAKAMURA
Article first published online: February 3, 2015
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In our previous study, we have reported inhibitory effects of flavonoids and benzo-furan type stilbenes from Brazilian herbal medicine, the aerial parts of Cissussicyoides, on antigen-induced degranulations in rat basophilic leukemia cells (RBL-2H3). In the present study, we examined effects of the active constituents on antigen-induced release of tumor necrosis factor-a (TNF-a) and interleukin-4 (IL-4) in the cells. The result revealed that flavonols (kaempferol, quercetin), flavones (lanceolatin B, 7,3',4'-trihydroxyflavone), chalcones (isoliquiritigenin, E-7-O-methylpongamol) inhibited the release of TNF-a and IL-4. With regard to the effects of active constituents on signaling transduction, these constituents (except for E-7-O-methylpongamol) suppressed the phosphorylation of ERK1/2, JNK/SAPK in the cells, andinhibition of spleen tyrosine kinase (Syk) and myocin light-chain kinase (MLCK) may, at least in part, be involved in the mechanism of action.
Keywords Flavonoids, Cissussicyoides, Cytokine, Target molecule, Spleen tyrosine kinase, Myocin light chain kinase